Chromosome mapping inCryptosporidium parvumand establishment of a long-range restriction map for chromosome VI
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چکیده
منابع مشابه
Refinement of human chromosome 7 map around the pro alpha 2(I)collagen gene by long-range restriction mapping.
The physical proximity of the closely linked pro alpha 2(1)collagen (COL1A2) and erythropoietin (EPO) genes and five loci with no known function was studied by long-range restriction mapping experiments using pulsed-field gel electrophoresis. COL1A2 and D7S64 were found to be within 100 kb of each other, providing a new informative marker for linkage studies with respect to COL1A2. D7S15 and D7...
متن کاملA long range restriction map of the distal human X chromosome short arm around the steroid sulfatase locus.
The distal short arm of the human X chromosome is of interest because it contains genes which escape X chromosome inactivation and because it is subject to frequent deletions in human patients. The steroid sulfatase gene has been particularly well studied as an example of a gene which escapes X inactivation and which is included in a number of these deletion events. For these reasons a physical...
متن کاملHuman chromosome 17 NotI linking clones and their use in long-range restriction mapping of the Miller-Dieker chromosome region (MDCR) in 17p13.3.
A NotI linking library constructed from flow-sorted human chromosome 17 material was screened to aid in construction of a long-range restriction map of the Miller-Dieker chromosome region (MDCR) in 17p13.3. A total of 66 clones were mapped to one of eight regions of chromosome 17 using a somatic cell hybrid panel, and 44/66 (67%) of these clones cross-hybridized to rodent DNA on Southern blots....
متن کاملHigh resolution restriction mapping of YACs using chromosome fragmentation.
The large insert size of yeast artificial chromosomes (YACs; 1) has made them ideal for the analysis of complex genomes. YACs can be restriction mapped with rare cutting enzymes but this does not provide the resolution required to investigate gene structure (such as locating exons and introns). Detailed mapping normally requires sub-cloning into cosmid or phage vectors. Here we describe a metho...
متن کاملPhysical map of a conditionally dispensable chromosome in Nectria haematococca mating population VI and location of chromosome breakpoints.
Certain isolates of the plant pathogenic fungus Nectria haematococca mating population (MP) VI contain a 1.6-Mb conditionally dispensable (CD) chromosome carrying the phytoalexin detoxification genes MAK1 and PDA6-1. This chromosome is structurally unstable during sexual reproduction. As a first step in our analysis of the mechanisms underlying this chromosomal instability, hybridization betwee...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1999.tb13625.x